Confocal Microscopy is a specialized form of fluorescent microscopy that uses particular optical components to generate high-resolution images of the material stained with a fluorescent probe. The main advantage of Confocal Microscopy is gathering quantitative data such as thickness, area and volume of cellular structure and promises to provide 3-D volume rendering of living cells and tissue over time.
This important facility was established at Advanced Centre for Plant Virology (Plant Virology Unit), IARIin 2012 under ICAR-Outreach Programme.
Model: Leica TCS SP52
Uses:
It is used in studying the physiology of host-pathogen interaction by
- Localization of proteins
- Characterization of structural proteins
- Characterization of fungal spores
- Live/dead cell counts
- Monitoring of virus infection in host and vector cells
Advantages:
- High-resolution blur-free images with enhanced picture quality with 3-D structures of living cells and tissues
- High depth of focus extended focus images without special object preparation
- Concurrent analysis of surface and internal structures
- Enables the examination of both living and fixed specimens under a variety of condition
More than 2000 samples including, viruses, fungi bacteria, and various plant samples (root/shoot/leaf/other plant parts) processed to date.
Processing charges:
Sl. No.
|
Organization
|
Rates per sample/specimen/slide
(in INR)
|
1
|
Government organizations (Other Divisions of IARI, Other Government Academic and Research Institutes)
|
Rs. 1500/- + GST
|
2
|
Private Industries and Profit Laboratories
|
Rs. 5000/- + GST
|
CONTACT US
Dr. Kajal Kumar Biswas
In-Charge, Plant Virology Unit
Division of Plant Pathology
ICAR-Indian Agricultural Research Institute
New Delhi – 110012
Mob: +91-9868796694
Contact Person
Dr. Shilpi Aggarwal
Technical Officer
Plant Virology Unit
Division of Plant Pathology
ICAR-Indian Agricultural Research Institute
New Delhi-110012
Mob: +91-9818191241